Lur'e L M, Bartoshevich Iu E, Levitov M M
Antibiotiki. 1980 Jan;25(1):3-9.
The attitude of the cephalosporin C-producing organism to various sources of carbon and nitrogen was studied. Carbohydrates such as maltose, starch and sucrose and nitrogen sources such as mineral ((NH4)2SO4 + KNO3) and organic nitrogen (asparagine) may be successfully used for the culture growth and antibiotic biosynthesis. The use of the mineral nitrogen necessitates additional regulation of pH during the cultivation process. The effect of pH on cephalosporin C biosynthesis was studied and its optimal values were found. It was shown that methyloleate played no specific role in cephalosporin C biosynthesis. It is an additional source of carbon which may be replaced by whale oil. It was demonstrated that the 1st stage of assimilation of inorganic sulfate, i.e. its reduction was blocked in C. acrimonium 218A. The study on the problem of phases in production of cephalosporin C showed that unlike the penicillin-producing organism C. acremonium had no capacity for the antibiotic biosynthesis at the early developmental stages irrespective of the cultivation conditions.
研究了产头孢菌素C的微生物对各种碳源和氮源的态度。麦芽糖、淀粉和蔗糖等碳水化合物以及矿物氮源（(NH4)2SO4 + KNO3）和有机氮源（天冬酰胺）可成功用于培养生长和抗生素生物合成。使用矿物氮需要在培养过程中额外调节pH值。研究了pH值对头孢菌素C生物合成的影响并找到了其最佳值。结果表明，油酸甲酯在头孢菌素C生物合成中没有特定作用。它是一种额外的碳源，可用鲸油替代。结果表明，顶头孢霉218A中无机硫酸盐同化的第一阶段，即其还原过程受阻。对头孢菌素C生产阶段问题的研究表明，与产青霉素的微生物不同，无论培养条件如何，顶头孢霉在发育早期都没有抗生素生物合成能力。
