Kozina A M, Maksimova E A, Bylinkina E S, Dmitrieva S V
Antibiotiki. 1982;27(9):649-54.
It was shown that intensification of cephalosporin biosynthesis is possible with the use of the inoculum grown on the seed medium enriched with the sources of carbon and organic nitrogen. The inoculum grown on the initial medium is usually added to the fermentation medium by the 31st-33rd hour of the growth, while with the use of the enriched medium it is added to the fermentation medium by the 36th-38th hour. The intensity of the culture respiration is recommended to be used as a technological parameter for estimation of the time when the inoculum may be added to the fermentation medium.
结果表明，使用在富含碳源和有机氮源的种子培养基上生长的接种物，可以增强头孢菌素的生物合成。在初始培养基上生长的接种物通常在生长的第31至33小时添加到发酵培养基中，而使用富集培养基时，则在第36至38小时添加到发酵培养基中。建议将培养物呼吸强度用作估计接种物可添加到发酵培养基中的时间的工艺参数。
