牛肝磷脂酰胆碱交换蛋白的亲和层析

Affinity chromatography of the phosphatidylcholine exchange protein from bovine liver.

作者信息

Barsukov L I, Dam C W, Bergelson L D, Muzja G I, Wirtz K W

出版信息

Biochim Biophys Acta. 1978 Nov 2;513(2):198-204. doi: 10.1016/0005-2736(78)90173-6.

Abstract

Affinity chromatography has been used to purify the phosphatidylcholine exchange protein from bovine liver. The affinity resin consisted of 1-acyl-2-(9-carboxy)nonyl-glycero-3-phosphocholine linked to AH-Sepharose 4 B via the carboxyl group. Application of a crude exchange protein fraction to the affinity column resulted in a complete adsorption of the phosphatidylcholine exchange protein. The exchange protein eluted with a buffer containing 0.15% sodium deoxycholate. The most active fraction was 130-fold purified and accounted for 62% of the activity.

摘要

亲和色谱法已被用于从牛肝中纯化磷脂酰胆碱交换蛋白。亲和树脂由通过羧基连接到琼脂糖凝胶4B上的1-酰基-2-（9-羧基）壬基甘油-3-磷酸胆碱组成。将粗交换蛋白组分应用于亲和柱导致磷脂酰胆碱交换蛋白完全吸附。交换蛋白用含有0.15%脱氧胆酸钠的缓冲液洗脱。活性最高的组分纯化了130倍，占总活性的62%。