Harrison E H, Smith J E, Goodman D S
Biochim Biophys Acta. 1980 Apr 3;628(4):489-97. doi: 10.1016/0304-4165(80)90397-9.
The levels of retinol-binding protein, prealbumin, and several 'marker' enzymes were determined in homogenates, crude subcellular fractions and isolated Golgi apparatus prepared from the livers of vitamin A-deficient and control rats. Vitamin A deficiency led to a marked increase (3.5-fold) in hepatic retinol-binding protein concentration and to slight increases in hepatic pre-albumin levels, without affecting the levels of a number of marker enzymes localized in various subcellular compartments. The distributions of total protein and marker enzymes among various subcellular fractions were nearly identical in control and vitamin A-deficient preparations. In particular, vitamin A deficiency had no effect on the yield or enzymatic composition of isolated Golgi-rich fractions. In vitamin A-deficient rats, where the normal secretion of retinol-binding protein was blocked, a maximum of less than 10% of the total liver retinol-binding protein was accounted for in the Golgi. In contrast, in control rats, where the secretion of retinol-binding protein was proceeding at the normal rate, the relative amount of retinol-binding protein in Golgi increased to about 23% of the total liver pool. The data suggest that the Golgi apparatus is involved in the pathway of retinol-binding protein secretion from the liver, but demonstrate that the Golgi is not the major subcellular locus for retinol-binding protein in either normal or vitamin A-deficient rats.
在从维生素A缺乏大鼠和对照大鼠肝脏制备的匀浆、粗亚细胞组分及分离的高尔基体中，测定了视黄醇结合蛋白、前白蛋白及几种“标记”酶的水平。维生素A缺乏导致肝脏视黄醇结合蛋白浓度显著升高（3.5倍），肝脏前白蛋白水平略有升高，而不影响定位于各种亚细胞区室的多种标记酶的水平。在对照和维生素A缺乏制剂中，总蛋白和标记酶在各种亚细胞组分中的分布几乎相同。特别是，维生素A缺乏对富含高尔基体组分的产量或酶组成没有影响。在维生素A缺乏的大鼠中，视黄醇结合蛋白的正常分泌受阻，高尔基体中占肝脏视黄醇结合蛋白总量的比例最高不到10%。相反，在对照大鼠中，视黄醇结合蛋白以正常速率分泌，高尔基体中视黄醇结合蛋白的相对量增加到肝脏总量的约23%。数据表明，高尔基体参与肝脏视黄醇结合蛋白的分泌途径，但表明在正常或维生素A缺乏的大鼠中，高尔基体都不是视黄醇结合蛋白的主要亚细胞定位场所。
