Smith J E, Deen D D, Sklan D, Goodman D S
J Lipid Res. 1980 Feb;21(2):229-37.
Studies were conducted to explore the effects of colchicine on the secretion and metabolism of retinol-binding protein (RBP) by the liver. In the vitamin A-deficient rat, the rate of secretion of RBP from the liver into the serum is greatly reduced, and RBP accumulates in the liver. Injection of retinol (dispersed in a 20% Tween 40 solution) into deficient rats stimulated a rapid secretion of RBP from the liver into the serum. Colchicine treatment markedly inhibited the retinol-stimulated secretion of RBP from the liver into the serum. The effect of colchicine was most pronounced during the early period after retinol injection, particularly during the first 30 to 60 minutes. Ninety minutes after retinol injection, the serum RBP level of colchicine treated rats was only 36% as great as that of the control rats. In parallel experiments, a quantitatively similar inhibition of very low density lipoprotein (VLDL) secretion by colchicine was observed in the retinol-deficient rats. In contrast, colchicine did not affect the overall rate of hepatic protein synthesis, as estimated from the incorporation of [(3)H]leucine into total liver and serum protein; the secretion of newly synthesized protein was, however, inhibited. When rats were treated with colchicine and then injected with retinol, the level of RBP in a Golgi-rich fraction obtained from the liver homogenate increased markedly as compared to the level of prealbumin. The inhibition of RBP secretion by colchicine suggests that the microtubules play a role in RBP secretion. By analogy to studies on VLDL and albumin, these data provide presumptive evidence that the Golgi apparatus and secretory vesicles are involved in RBP secretion.-Smith, J. E., D. D. Deen, Jr., D. Sklan, and D. S. Goodman. Colchicine inhibition of retinol-binding protein secretion by rat liver.
开展了多项研究以探究秋水仙碱对肝脏视黄醇结合蛋白（RBP）分泌及代谢的影响。在维生素A缺乏的大鼠中，肝脏向血清中分泌RBP的速率大幅降低，且RBP在肝脏中蓄积。向缺乏维生素A的大鼠注射视黄醇（分散于20%吐温40溶液中）可刺激肝脏快速向血清中分泌RBP。秋水仙碱处理显著抑制了视黄醇刺激的肝脏向血清中分泌RBP的过程。秋水仙碱的作用在视黄醇注射后的早期最为明显，尤其是在最初的30至60分钟内。视黄醇注射90分钟后，经秋水仙碱处理的大鼠血清RBP水平仅为对照大鼠的36%。在平行实验中，在视黄醇缺乏的大鼠中观察到秋水仙碱对极低密度脂蛋白（VLDL）分泌有类似程度的抑制作用。相比之下，根据[³H]亮氨酸掺入肝脏总蛋白和血清蛋白的情况估算，秋水仙碱并不影响肝脏蛋白质合成的总体速率；然而，新合成蛋白质的分泌受到了抑制。当大鼠用秋水仙碱处理后再注射视黄醇时，从肝脏匀浆中获得的富含高尔基体部分中的RBP水平与前白蛋白水平相比显著升高。秋水仙碱对RBP分泌的抑制表明微管在RBP分泌中起作用。通过与对VLDL和白蛋白的研究进行类比，这些数据提供了推测性证据，表明高尔基体和分泌囊泡参与了RBP的分泌。-史密斯，J. E.，D. D. 迪恩，Jr.，D. 斯克兰，和D. S. 古德曼。秋水仙碱对大鼠肝脏视黄醇结合蛋白分泌的抑制作用。
