Thermal denaturation of distamycin A--DNA complexes as followed by hyperchromic spectra.

Interaction of distamycin A with calf spleen DNA is investigated by the method of hyperchromic spectra. Hyperchromic spectra of complexes are partitioned into the components corresponding to the denaturation A . T and G . C base pairs and dissociation of the ligand, fractions of respective components are found as a function of temperature. A scheme of melting of successive regions of DNA with different G+C content together with the scheme of distamycin A redistribution in the course of thermal denaturation is presented.