Yang Y T, Williams M A
Biochim Biophys Acta. 1978 Nov 22;531(2):133-40. doi: 10.1016/0005-2760(78)90136-4.
C18-, C20- and C22-unsaturated acids were tested for inhibition of fatty acid synthesis in hepatocytes isolated from essential fatty acid-deficient rats. Fatty acid synthesis was measured by incorporation of radioactivity from [1-14C-A1-acetate or 3H2O into fatty acids. C20-polyunsaturated fatty acids included arachidonic acid (20 : 4 (n-6)) and 4 other fatty acids formed from linoleic acid (18 : 2 (n-6)) or linolenic acid (18 : 3 (n-3)). These were (11,14)-icosadienoic acid (20 : 2 (n-6)), (8,11,14)-icosatrienoic acid (20 : 3 (n-6)), (11,14,17)-icosatrienoic acid (20 : 3 (n-3)) and (5,8,11,14,17)-icosapentaenoic acid (20 : 5 (n-3). All of these have essential fatty acid activity. The fatty acid (5,8,11)-icosatrienoic acid (20 : 3 (n-9)) was also tested. This fatty acid is formed from oleic acid (18 : 1 (n-9)) and is not an essential fatty acid or a prostaglandin precursor. C20-unsaturated fatty acids and (22 : 6 (n-3)) were as effective as stearic acid in inhibiting fatty acid synthesis and were more inhibitory than their precursor C18-unsaturated fatty acids. These results are evidence that C20-unsaturated fatty acids of the linoleic and linolenic acid series can act in short-term inhibition as well as in adaptive inhibition of fatty acid synthesis (Bloch, K. and Vance, D. (1977) Annu. Rev. Biochem. 46, 263--298). The effectiveness of (5,8,11)-icosatrienoic acid indicates that short-term inhibition by C20-unsaturated fatty acids is not limited to those fatty acids which have essential fatty acid activity.
对C18 -、C20 -和C22 -不饱和酸进行了测试，以研究它们对从必需脂肪酸缺乏大鼠分离出的肝细胞中脂肪酸合成的抑制作用。脂肪酸合成通过将[1 - 14C]乙酸盐或3H2O中的放射性掺入脂肪酸来测定。C20 -多不饱和脂肪酸包括花生四烯酸（20 : 4 (n - 6)）以及由亚油酸（18 : 2 (n - 6)）或亚麻酸（18 : 3 (n - 3)）形成的其他4种脂肪酸。它们分别是（11,14）-二十碳二烯酸（20 : 2 (n - 6)）、（8,11,14）-二十碳三烯酸（20 : 3 (n - 6)）、（11,14,17）-二十碳三烯酸（20 : 3 (n - 3)）和（5,8,11,14,17）-二十碳五烯酸（20 : 5 (n - 3)）。所有这些都具有必需脂肪酸活性。还测试了脂肪酸（5,8,11）-二十碳三烯酸（20 : 3 (n - 9)）。这种脂肪酸由油酸（18 : 1 (n - 9)）形成，不是必需脂肪酸或前列腺素前体。C20 -不饱和脂肪酸和（22 : 6 (n - 3)）在抑制脂肪酸合成方面与硬脂酸一样有效，并且比它们的前体C18 -不饱和脂肪酸更具抑制作用。这些结果证明，亚油酸和亚麻酸系列的C20 -不饱和脂肪酸在脂肪酸合成的短期抑制以及适应性抑制中都能发挥作用（布洛赫，K.和万斯，D.（1977年）《生物化学年度评论》46，263 - 298）。（5,8,11）-二十碳三烯酸的有效性表明，C20 -不饱和脂肪酸的短期抑制作用并不局限于那些具有必需脂肪酸活性的脂肪酸。
