Somorová M, Frimm R, Welward L, Dudek M, Lukasiková E
Antibiotiki. 1980 May;25(5):330-3.
During fermentation of Acremonium chrysogenum, known cephalosporin-producing organism, there is formed, in addition, the main metabolite cephalosporin C. Its 3-desacetyl derivative, desacetylcephalosporin (DACS). The admixture of this derivative is not desirable. Such factors as the value of pH aeration level and concentration of free glucose influence formation of DACS. A method of descending chromatography with the use of Whatman paper No. 4 and the system of n-butanol-acetic acid-water (60:15:25) was elaborated for separation of the cephalosporins. The spot of DACS with Rf 0.15 was developed in UV light at 254 nm and eluated with phosphate buffer. The eluate absorption was estimated at a wave length of 260 nm and the amount of DACS was determining by the standard curve. The effect of various fermentation conditions on formation of DACS was compared. The highest levels of DACS was compared. The highest levels of DACS formation were observed in 500 ml flasks with unfavourable aeration and agitation conditions. Determination of the DACS levels during the fermentation process provides control of pH, aeration rate and sugar levels which results in minimum formation of DACS. Close chemical structures of DACS and cephalosporin C impede their separation during the process of cephalosporin C recovery.
在产头孢菌素的顶头孢霉发酵过程中，除了主要代谢产物头孢菌素C外，还会形成其3 - 去乙酰基衍生物去乙酰头孢菌素（DACS）。这种衍生物的混合是不理想的。诸如pH值、通气水平和游离葡萄糖浓度等因素会影响DACS的形成。已精心设计了一种使用Whatman 4号滤纸和正丁醇 - 乙酸 - 水（60:15:25）体系的下行色谱法来分离头孢菌素。Rf为0.15的DACS斑点在254 nm紫外光下显色，并用磷酸盐缓冲液洗脱。在260 nm波长下估计洗脱液的吸光度，通过标准曲线测定DACS的量。比较了各种发酵条件对DACS形成的影响。比较了DACS的最高水平。在通气和搅拌条件不利的500 ml烧瓶中观察到DACS形成的最高水平。在发酵过程中测定DACS水平可控制pH值、通气速率和糖水平，从而使DACS的形成降至最低。DACS和头孢菌素C紧密的化学结构阻碍了它们在头孢菌素C回收过程中的分离。
