[Study of the structure and aggregation of histones. II. Structure of oligomers forming in mixed solutions of histones H2A and H4].

A change of light-scattering and sedimentation constant of histones H2A and H4 of monomers and oligomers has been studied. It has been shown that in salt-free solution the histone monomers are present. There is a mixture of oligomers (H2A)3, S20,w = 3,8S; (H2A)-(H4)1, S20,w = 3,8S; (H4)60, S20,w = 27S in the solution 0,6 M NaCl depending on fractions correlation. It has been shown that the molecules interactions in the composition of oligomers are cooperative and the cross interactions block the histone H4 self-aggregation.