[组蛋白的结构与聚集研究。II. H2A和H4组蛋白混合溶液中形成的寡聚体结构]
[Study of the structure and aggregation of histones. II. Structure of oligomers forming in mixed solutions of histones H2A and H4].
作者信息
Khrapunov S N, Berdyshev G D, Dragan A I
出版信息
Biofizika. 1978 Nov-Dec;23(6):995-9.
A change of light-scattering and sedimentation constant of histones H2A and H4 of monomers and oligomers has been studied. It has been shown that in salt-free solution the histone monomers are present. There is a mixture of oligomers (H2A)3, S20,w = 3,8S; (H2A)-(H4)1, S20,w = 3,8S; (H4)60, S20,w = 27S in the solution 0,6 M NaCl depending on fractions correlation. It has been shown that the molecules interactions in the composition of oligomers are cooperative and the cross interactions block the histone H4 self-aggregation.
对组蛋白H2A和H4单体及寡聚体的光散射和沉降常数变化进行了研究。结果表明，在无盐溶液中存在组蛋白单体。在0.6M NaCl溶液中，根据组分相关性，存在寡聚体混合物：(H2A)3，S20,w = 3.8S；(H2A)-(H4)1，S20,w = 3.8S；(H4)60，S20,w = 27S。结果表明，寡聚体组成中的分子相互作用是协同的，交叉相互作用阻止了组蛋白H4的自我聚集。
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