Hoover R L, Bhalla D K, Yanovich S, Inbar M, Karnovsky M J
J Cell Physiol. 1980 Jun;103(3):399-406. doi: 10.1002/jcp.1041030305.
The incubation of linoleic acid with cells causes profound effects on membrane asociated phenomenon. Using the fluorescent probe diphenyl hexatriene (DPH) to monitor lipid changes in the microenvironment of the cell surface, we find that linoleic acid reduces the polarization values (P) in mouse lymphocytes and BHK cells. Measurements on lipids extracted from the cells grown in linoleic acid produce similar results. We also find in the mouse lymphocyte that capping of Ig is inhibited and con A stimulated mitogenesis is unaffected. In contrast to the latter effect, LPS and PHA stimulated mitogenesis is inhibited and in the rat lymph node, con A stimulated mitogenesis, greatly enhanced. We also show that linoleic acid alters the binding of antibodies to the cell surface of EL-4 lymphoma cells. These observations suggest that linoleic acid alters cellular function by interfering with protein/lipid interactions within the surface membrane.
亚油酸与细胞一起温育会对膜相关现象产生深远影响。使用荧光探针二苯基己三烯（DPH）来监测细胞表面微环境中的脂质变化，我们发现亚油酸会降低小鼠淋巴细胞和BHK细胞中的极化值（P）。对在亚油酸中生长的细胞提取的脂质进行测量也产生了类似结果。我们还发现在小鼠淋巴细胞中，Ig的帽化受到抑制，而伴刀豆球蛋白A刺激的有丝分裂不受影响。与后一种效应相反，脂多糖和PHA刺激的有丝分裂受到抑制，而在大鼠淋巴结中，伴刀豆球蛋白A刺激的有丝分裂大大增强。我们还表明，亚油酸会改变抗体与EL-4淋巴瘤细胞表面的结合。这些观察结果表明，亚油酸通过干扰表面膜内的蛋白质/脂质相互作用来改变细胞功能。
