Wildt D E, Seager S W, Chakraborty P K
Endocrinology. 1980 Oct;107(4):1212-7. doi: 10.1210/endo-107-4-1212.
The relationship among the number of copulatory stimuli, the incidence of ovulation, and the pituitary release of LH was studied in the female cat (queen), an induced or reflex-ovulating species. Estrual queens were mated with a male either once during estrus (on day 1, 2, 3, or 4) or three times at 4-h intervals in a single day of estrus (on day 1, 2, or 3). Laparoscopy was used to examine the ovaries, initially to determine the numbers of preovulatory follicles and later to count corpora lutea. Blood samples were obtained serially from cats in each mating group, and sera were analyzed for LH. The day of estrus on which mating occurred, estrus duration, and follicle numbers had no effect on the proportion of animals ovulating. A single mating resulted in fewer queens ovulating per group (10 of 48 animals) than three matings on a single day (30 of 36). After copulation, LH was elevated only in animals later confirmed to ovulate. LH response in ovulating queens varied among individuals but, when averaged, appeared to be greater and more prolonged in multiple mated vs. single mated animals. These data indicate that the reflex-ovulating cate does not always ovulate after a single copulatory stimulus because of a failure of LH release. The frequency of coital stimulation apparently plays a major role in influencing the proportion of animals ovulating, with multiple copulation apparently enhancing the pituitary release of LH.
在诱导排卵或反射排卵的雌性猫（母猫）中，研究了交配刺激次数、排卵发生率与垂体促黄体生成素（LH）释放之间的关系。处于发情期的母猫在发情期（第1、2、3或4天）与雄性交配一次，或在发情期的一天内（第1、2或3天）每隔4小时交配三次。使用腹腔镜检查卵巢，最初是为了确定排卵前卵泡的数量，后来是为了统计黄体数量。从每个交配组的猫身上连续采集血样，并分析血清中的LH。交配发生的发情日、发情持续时间和卵泡数量对排卵动物的比例没有影响。单次交配导致每组排卵的母猫数量（48只动物中的10只）少于同一天的三次交配（36只动物中的30只）。交配后，LH仅在后来证实排卵的动物中升高。排卵母猫的LH反应在个体之间有所不同，但平均而言，多次交配的动物与单次交配的动物相比，LH反应似乎更大且更持久。这些数据表明，由于LH释放失败，反射排卵的母猫在单次交配刺激后并不总是排卵。性交刺激的频率显然在影响排卵动物的比例方面起主要作用，多次交配显然会增强垂体LH的释放。
