Kalish R, Brody N I
J Invest Dermatol. 1980 Sep;75(3):275-8. doi: 10.1111/1523-1747.ep12523542.
The injection of either viable B16 melanoma cells, killed B16 cells, or B16 cell products increased the incidence of melanomas in C57Bl/6J mice inoculated with a threshold dose of B16 cells. In all cases the effect was seen whether the facilitating injection was at a site distant from the challenge inoculum or at the same site. Facilitation was seen with B16 cells and products both from in vivo tumors and from tissue culture. However, cells detached from tissue culture flasks with trypsin no longer had facilitating activity. Facilitating activity was found in concentrated cultured supernatants centrifuged at 100,000 X g for 1.5 hr and dialyzed. No activity was detected in the less than 10,000 mol wt fraction. Facilitation was not associated with a change in the time of tumor appearance nor with enhanced growth of B16 cells in culture.
注射活的B16黑色素瘤细胞、灭活的B16细胞或B16细胞产物，可增加接种阈值剂量B16细胞的C57Bl/6J小鼠黑色素瘤的发生率。在所有情况下，无论促进注射是在远离激发接种物的部位还是在同一部位，均可见到这种效应。来自体内肿瘤和组织培养的B16细胞及产物均可见促进作用。然而，用胰蛋白酶从组织培养瓶中分离的细胞不再具有促进活性。在以100,000×g离心1.5小时并透析的浓缩培养上清液中发现了促进活性。在分子量小于10,000的部分未检测到活性。促进作用与肿瘤出现时间的变化无关，也与培养中B16细胞的生长增强无关。
