Hübschle O J, Weiland F
Zentralbl Bakteriol A. 1980 Mar;246(2):141-50.
Several methods of coupling immunoglobulins onto sheep erythrocytes have been examined with a view to demonstrate Semliki-Forest virus by use of a passive hemagglutination tests. With the exception of the carbodiimid coupling reaction, most methods could be used to couple goat anti SFV IgG to erythrocytes. Best results were obtained when anti SFV goat IgG was coupled to sheep red blood cells by using 0.25% glutaraldehyde. Sheep red blood cells treated after that method could be used to identify as well as titrate SFV in any given solution. The actual mode of agglutination was made visible by electron microscopy in so far as SFV was found to form dimers of adjacent erythrocytes via a bridge formation. The passive hemagglutination offers considerable advantages compared to other methods such as the direct hemagglutination with goose erythrocytes, as formalin inactivated virus can be detected therby. The described test therefore offers an additional approach to check the potency of inactivated togavirus vaccines.
为了通过被动血凝试验检测塞姆利基森林病毒，已对几种将免疫球蛋白偶联到绵羊红细胞上的方法进行了研究。除了碳二亚胺偶联反应外，大多数方法都可用于将山羊抗塞姆利基森林病毒IgG偶联到红细胞上。当使用0.25%戊二醛将抗塞姆利基森林病毒山羊IgG偶联到绵羊红细胞上时，可获得最佳结果。用该方法处理后的绵羊红细胞可用于鉴定和滴定任何给定溶液中的塞姆利基森林病毒。通过电子显微镜观察到实际的凝集模式，因为发现塞姆利基森林病毒通过桥连形成相邻红细胞的二聚体。与其他方法如用鹅红细胞进行直接血凝相比，被动血凝具有相当大的优势，因为由此可以检测到福尔马林灭活病毒。因此，所描述的试验为检查灭活披膜病毒疫苗的效力提供了另一种方法。
