Do U H, Ramachandran S
J Lipid Res. 1980 Sep;21(7):888-94.
Chicken egg yolk phospholipids were subjected to mild alkaline hydrolysis. The resulting alkali-stable phospholipids were characterized by chemical chromatographic, and enzymatic methods. Two major phospholipids, 1 - O - alkyl - sn - glycero - 3 - pohosphoethanolamine and sphingomyelin; and two minor phospholipids, 1-O-alkenyl-sn-glycero-3-phosphoethanolamine and 1-O-alkyl-sn-glycero-3-phosphocholine; were identified. The sphingomyelins were converted into ceramides by enzymatic hydrolysis with phospholipase C. Ceramides derived from sphingomyelins with non-hydroxy fatty acids (99% of total ceramides) consisted predominantly of the N-palmitoyl, N-stearoyl, and N-nervonyl species. Ceramides derived from sphingomyelins with hydroxy fatty acids (1% of total ceramides) consisted almost exclusively of the N-alpha-hydroxyeicosanoyl species. The long chain bases of ceramides derived from both species of sphingomyelins (hydroxy and non-hydroxy fatty acids) consisted of 97% D-erythro-sphing-4-enine and 3% D-erythro-sphinganine, 1-O-alkyl-sn-glycero-3-phosphoethanolamine and 1-O-alkyl-sn-glycero-3-phosphocholine were converted to corresponding 1-O-alkyldiacetylglycerols. The 1-O-alkyldiacetylglycerols derived from both ether phospholipids consisted largely of the hexadecyl and octadecyl species. Smaller quantities of the heptadecyl, cis-9-octadecenyl and eicosanyl derivatives were also present.
鸡卵黄磷脂进行温和碱性水解。通过化学、色谱和酶法对所得的碱稳定磷脂进行表征。鉴定出两种主要磷脂，1-O-烷基-sn-甘油-3-磷酸乙醇胺和鞘磷脂；以及两种次要磷脂，1-O-烯基-sn-甘油-3-磷酸乙醇胺和1-O-烷基-sn-甘油-3-磷酸胆碱。鞘磷脂通过磷脂酶C的酶促水解转化为神经酰胺。源自含非羟基脂肪酸的鞘磷脂的神经酰胺（占总神经酰胺的99%）主要由N-棕榈酰、N-硬脂酰和N-神经酰基种类组成。源自含羟基脂肪酸的鞘磷脂的神经酰胺（占总神经酰胺的1%）几乎完全由N-α-羟基二十碳酰基种类组成。源自两种鞘磷脂（羟基和非羟基脂肪酸）的神经酰胺的长链碱基由97%的D-赤藓糖神经鞘氨醇-4-烯和3%的D-赤藓糖神经鞘氨醇组成，1-O-烷基-sn-甘油-3-磷酸乙醇胺和1-O-烷基-sn-甘油-3-磷酸胆碱转化为相应的1-O-烷基二乙酰甘油。源自两种醚磷脂的1-O-烷基二乙酰甘油主要由十六烷基和十八烷基种类组成。还存在少量的十七烷基、顺-9-十八碳烯基和二十烷基衍生物。
