[关于用二乙氨基乙基纤维素测定性激素结合球蛋白的方法学问题]

[Methodological problems concerning the assay of sex hormone binding globulin with DEAE-cellulose].

作者信息

Caputo M, Croci R, Chimini G, Biassoni P, Bagnasco M

出版信息

Boll Soc Ital Biol Sper. 1980 Jul 30;56(14):1485-91.

PMID:7192563

Abstract

Since 1966 a carrier protein for the sex hormones, testosterone and estradiol, has been identified. Mickelson and Petra measured SHBG concentration by adsorption method in 1974. The SHBG contained in serum is incubated with DHT-3H and adsorbed to a cellulose filter. In this study we have reviewed this method. The method showed good reproducibility with a coefficient of variation of 8,1% for low concentration of SHBG and 11,6% for high concentration. No effects of serum dilution was observed. Normal values, expressed as micrograms DHT bound in 100 ml serum, resulted: females, 0,88 +/- 0,08 (ES); males, 0,58 +/- 0,04; pregnancy, 2,94 +/- 0,4. The mean value for males was significantly lower than the mean value for normal females (P < 0,005); pregnancy had a mean value statistically different from the normal females (P < 0,001).

摘要

自1966年以来，已鉴定出一种性激素睾酮和雌二醇的载体蛋白。1974年，米克尔森和佩特拉通过吸附法测量了性激素结合球蛋白（SHBG）的浓度。将血清中所含的SHBG与3H双氢睾酮（DHT）一起孵育，然后吸附到纤维素滤膜上。在本研究中，我们回顾了该方法。该方法显示出良好的重现性，低浓度SHBG的变异系数为8.1%，高浓度SHBG的变异系数为11.6%。未观察到血清稀释的影响。以每100 ml血清中结合的DHT微克数表示的正常值如下：女性为0.88±0.08（ES）；男性为0.58±0.04；孕期为2.94±0.4。男性的平均值显著低于正常女性的平均值（P<0.005）；孕期的平均值与正常女性在统计学上有差异（P<0.001）。