Claussen U, Krengel H G, Schrörs H J
Arzneimittelforschung. 1980;30(9):1585-8.
On the 9th day of gestation of rabbits the embryotoxic effects of cyclophosphamide were tested in vivo by i.v. injection (20, 40 and 80 mg/kg) and by injection into the yolk sac of rabbit embryos (20 micrograms, 80 micrograms 320 micrograms/blastoderm; yolk-sac-method). Furthermore pharmacological studies were undertaken to investigate cyclophosphamide and its metabolism in the blastoderm (york sac fluid and embryo). When applied by i.v. injection, cyclophosphamide was embryolethal and teratogenic in a dose-dependent manner. No embryotoxic effects could be observed by injection of unchanged cyclophosphamide into the yolk sac of rabbit embryos. Pharmacokinetic studies did not show any metabolism in the blastoderm by injection or unchanged cyclophosphamide into the yolk sac. The embryotoxicity of unchanged cyclophosphamide is discussed.
在兔妊娠第9天，通过静脉注射（20、40和80毫克/千克）以及向兔胚胎卵黄囊注射（20微克、80微克、320微克/胚盘；卵黄囊法），在体内测试环磷酰胺的胚胎毒性作用。此外，还进行了药理学研究，以研究环磷酰胺及其在胚盘（卵黄囊液和胚胎）中的代谢情况。当通过静脉注射给药时，环磷酰胺具有胚胎致死性和剂量依赖性致畸性。向兔胚胎卵黄囊注射未改变的环磷酰胺未观察到胚胎毒性作用。药代动力学研究未显示通过向卵黄囊注射未改变的环磷酰胺在胚盘中有任何代谢。讨论了未改变的环磷酰胺的胚胎毒性。
