Denk H, Franke W W
Eur J Cell Biol. 1981 Feb;23(2):241-9.
Large cytoplasmic aggregates (Mallory bodies) containing randomly arranged 10 to 20 nm thick filaments are characteristic structures of hepatocytes in certain liver diseases, especially alcoholic hepatitis of man and griseofulvin intoxication of mouse. Biochemical and immunological studies have shown that such Mallory bodies contain structural proteins related to the cytokeratins present in epithelial cells of various kinds, normal hepatocytes included. We have studied the mode of reorganization of the hepatocyte cytoskeleton during involution of Mallory bodies experimentally induced in mice by prolonged griseofulvin feeding after withdrawal of the drug, using immunofluorescence microscopy and electron microscopy. Dispersal of Mallory bodies into small granules accumulating in the hepatocyte periphery is a characteristic feature of Mallory body involution. Dispersed heaps of Mallory body material are seen in hepatocyte cortices at lateral (hepatocyte-to-hepatocyte) as well as at sinusoidal cell surfaces. Mallory body material dispersed to the cell periphery exhibits a filamentous ultrastructure an often shows conspicuous associations with desmosomes. Three different types of desmosome complexes can be discriminated in livers of mice allowed to recover from the intoxication: (i) desmosomes with normally looking bundles of tonofilaments; (ii) desmosomes associated with Mallory body filaments but few, if any, tono-filaments; and (iii) desmosomes lacking tonofilaments as well as Mallory body filaments. The associations between Mallory body filaments and desmosomes during Mallory body involution further strengthen the relationship between Mallory body filaments and tonofilaments. The observations suggest that Mallory body material, at least in part, may be utilized for rearrangement of the hepatocyte cytoskeleton, especially tonofilament-desmosome complexes.
大型细胞质聚集体（马洛里小体）含有随机排列的10至20纳米厚的细丝，是某些肝脏疾病中肝细胞的特征性结构，尤其是人类酒精性肝炎和小鼠灰黄霉素中毒。生化和免疫学研究表明，这种马洛里小体含有与各种上皮细胞（包括正常肝细胞）中存在的细胞角蛋白相关的结构蛋白。我们使用免疫荧光显微镜和电子显微镜，研究了在给小鼠长期喂食灰黄霉素后停药，实验诱导产生的马洛里小体退化过程中肝细胞细胞骨架的重组模式。马洛里小体分散成积聚在肝细胞周边的小颗粒是马洛里小体退化的一个特征。在肝细胞侧面（肝细胞与肝细胞之间）以及窦状细胞表面的肝细胞皮质中可见分散的马洛里小体物质堆。分散到细胞周边的马洛里小体物质呈现丝状超微结构，并且常常与桥粒有明显的联系。在从中毒中恢复的小鼠肝脏中可以区分出三种不同类型的桥粒复合体：（i）具有外观正常的张力丝束的桥粒；（ii）与马洛里小体细丝相关但几乎没有张力丝的桥粒；（iii）既没有张力丝也没有马洛里小体细丝的桥粒。在马洛里小体退化过程中，马洛里小体细丝与桥粒之间的联系进一步加强了马洛里小体细丝与张力丝之间的关系。这些观察结果表明，马洛里小体物质至少部分可用于肝细胞细胞骨架的重排，尤其是张力丝 - 桥粒复合体。
