Davison P F, Jones R N
J Cell Biol. 1981 Jan;88(1):67-72. doi: 10.1083/jcb.88.1.67.
Several classes of 10-nm filaments have been reported in mammalian cells and they can be distinguished by the size of their protein subunit. We have studied the distribution of these filaments in nerves from calves and other mammals. From the display on polyacrylamide electrophoretic gels of proteins in extracts from fibroblast and central, cranial and peripheral nerves, we cut the appropriate stained bands and prepared iodinated peptide maps. The similarities between the respective maps provide strong evidence for the presence of vimentin in cranial and peripheral nerves. The glial fibrillary acidic protein was found in axon preparations from the central nervous system, but was not identified in distal segments of some cranial nerves, nor in peripheral nerve.
在哺乳动物细胞中已报道了几类10纳米的细丝,它们可根据其蛋白质亚基的大小加以区分。我们研究了这些细丝在小牛及其他哺乳动物神经中的分布情况。通过对成纤维细胞、中枢神经、颅神经和外周神经提取物中的蛋白质进行聚丙烯酰胺电泳凝胶展示,我们切下适当的染色条带并制备了碘化肽图谱。各图谱之间的相似性为颅神经和外周神经中波形蛋白的存在提供了有力证据。在中枢神经系统的轴突制剂中发现了胶质纤维酸性蛋白,但在一些颅神经的远端节段以及外周神经中未鉴定到。