Bennell M A, Husband A J
Res Vet Sci. 1981 May;30(3):353-6.
Experiments are described which demonstrate that intraperitoneal injection of a soluble protein antigen (ovalbumin) in Freund's complete adjuvant (FCA) followed 14 days later by seven days of repeated infusions of an ovalbumin/DEAE-dextran mixture resulted in the appearance of IgA-specific antiovalbumin-containing cells (AOCC) in the intestinal lamina propria and IgA associated specific antibody in intestinal secretion of pigs. Neither intraperitoneal injection alone nor infusion of the antigen mixture alone resulted in a significant local intestinal immune response. In intraperitoneal primed animals the repeated infusion of ovalbumin without DEAE-dextran or a single intraduodenal injection of ovalbumin both resulted in a similar AOCC response in the intestine but, in the absence of DEAE-dextran, IgA was not the predominant class specificity of AOCC. Evidence is also presented for a depression of the intestinal immune response when intraperitoneal immunisation was performed using a large dose of antigen in FCA.
文中描述了一些实验，这些实验表明，在弗氏完全佐剂（FCA）中腹腔注射可溶性蛋白抗原（卵清蛋白），14天后再连续7天重复输注卵清蛋白/二乙氨基乙基葡聚糖混合物，会导致猪的肠固有层出现含IgA特异性抗卵清蛋白细胞（AOCC），并在肠道分泌物中出现与IgA相关的特异性抗体。单独腹腔注射或单独输注抗原混合物均未引发显著的局部肠道免疫反应。在经腹腔启动免疫的动物中，重复输注不含二乙氨基乙基葡聚糖的卵清蛋白或单次十二指肠内注射卵清蛋白，均可在肠道中引发类似的AOCC反应，但在没有二乙氨基乙基葡聚糖的情况下，IgA并非AOCC的主要类别特异性。文中还提供了证据，表明当在FCA中使用大剂量抗原进行腹腔免疫时，肠道免疫反应会受到抑制。
