McCrae M A, Woodland H R
Eur J Biochem. 1981 Jun 1;116(3):467-70. doi: 10.1111/j.1432-1033.1981.tb05359.x.
The naturally non-polyadenylated mRNAs of reovirus were shown to have a half-life in excess of 3 days when injected into Xenopus laevis oocytes. Hybridization analysis and gel electrophoresis showed that all 10 mRNA species had a similar high stability, despite being translated at widely differing rates. We have confirmed previous findings indicating a role for the 5'-terminal cap structure in determining reovirus mRNA stability [Furuichi et al. (1977) Nature (Lond.) 266, 235-239]. The significance of these results in relation to a general role for poly(A) in messenger RNA function is discussed.
将呼肠孤病毒天然的非聚腺苷酸化mRNA注射到非洲爪蟾卵母细胞中时，其半衰期超过3天。杂交分析和凝胶电泳表明，尽管10种mRNA的翻译速率差异很大，但它们都具有相似的高稳定性。我们证实了先前的研究结果，即5'-末端帽结构在决定呼肠孤病毒mRNA稳定性方面发挥作用[古市等（1977年），《自然》（伦敦），第266卷，第235 - 239页]。本文讨论了这些结果对于聚腺苷酸在信使RNA功能中的一般作用的意义。
