Gil A, Sanchez-Medina F
J Dairy Res. 1981 Feb;48(1):35-44. doi: 10.1017/s0022029900021427.
The acid-soluble ribonucleotides of cow's, goat's and sheep's milks were measured by enzymic and ion-exchange chromatographic procedures at different stages of lactation. Colostra and milk from ruminant species contained orotic acid and 13 well-identified nucleotides: AMP, CMP, GMP, UMP, UDP, GDP, UDP-glucose, UDP-galactose, UDP-N-acetyl-glucosamine, UDP-N-acetyl-galactosamine, UDP-glucuronate, GDP-mannose and GDP=fucose. Cow's goat's and sheep's colostrum contained significant amounts of nucleotides, which increased in amount from the moment of parturition, to reach a maximum 24--48 h later. The nucleotide concentration decreased thereafter with advancing lactation. Cow's milk contained substantial amounts of orotic acid, which increased during lactation, whereas in goat's and sheep's milk no increase took place. The cytidine and adenosine derivatives in ruminants' milk did not change significantly during lactation. The UDP-glucose/UDP-galactose ratio was measured in ruminants' milk at different stages of lactation.
采用酶法和离子交换色谱法，对奶牛、山羊和绵羊在泌乳不同阶段的乳中酸溶性核糖核苷酸进行了测定。反刍动物的初乳和乳中含有乳清酸和13种已明确鉴定的核苷酸：AMP、CMP、GMP、UMP、UDP、GDP、UDP - 葡萄糖、UDP - 半乳糖、UDP - N - 乙酰葡糖胺、UDP - N - 乙酰半乳糖胺、UDP - 葡糖醛酸、GDP - 甘露糖和GDP - 岩藻糖。奶牛、山羊和绵羊的初乳中含有大量核苷酸，自分娩时起数量增加，在24 - 48小时后达到最大值。此后，随着泌乳期推进，核苷酸浓度降低。牛奶中含有大量乳清酸，其在泌乳期间增加，而山羊奶和绵羊奶中则没有增加。反刍动物乳中的胞苷和腺苷衍生物在泌乳期间没有显著变化。在反刍动物泌乳的不同阶段对其乳中的UDP - 葡萄糖/UDP - 半乳糖比值进行了测定。
