单层细胞培养物的原位液体丙烷喷射冷冻和冷冻蚀刻
In situ liquid propane jet-freezing and freeze-etching of monolayer cell cultures.
作者信息
Espevik T, Elgsaeter A
出版信息
J Microsc. 1981 Jul;123(Pt 1):105-10. doi: 10.1111/j.1365-2818.1981.tb01284.x.
Abstract
A procedure for in situ liquid propane jet-freezing of unfixed and unglycerinated monolayer cell cultures is described. We have used the method to study monolayer cultures of rat Sertoli cells and human monocytes. The described procedure gives a cooling rate during specimen freezing high enough to yield adequately frozen specimens even in the absence of any cryoprotectant. Use of the procedure therefore eliminates artefacts usually caused by cell fixation and addition of cryoprotectives.
摘要
本文描述了一种对未固定、未甘油处理的单层细胞培养物进行原位液体丙烷喷射冷冻的方法。我们已使用该方法研究大鼠支持细胞和人单核细胞的单层培养物。所描述的方法在标本冷冻过程中提供了足够高的冷却速率，即使在没有任何冷冻保护剂的情况下也能产生充分冷冻的标本。因此，使用该方法可消除通常由细胞固定和添加冷冻保护剂引起的假象。
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