Jakob W, Heder G, Jentzsch K D
Exp Pathol. 1981;19(2):130-5. doi: 10.1016/s0232-1513(81)80043-6.
Porcine and bovine corpora lutea contain an activity capable of stimulating the initiation of DNA synthesis of mouse embryo fibroblasts, calf aorta media cells, and calf aorta endothelial cells. After fractionation of extracts produced by gradual ammonium sulfate precipitation by means of ion exchange chromatography on CM-Sephadex the activity is especially enriched in the last fraction (peak 5). The initiation of the DNA synthesis of calf aorta endothelial cells, however, is strongly dependent on the concentration of the labelled thymidine in the incubation medium. Calf aorta endothelial cells significantly respond to this corpus luteum factor only at a reduced 3H-thymidine concentration of 9.25 nM. At a 3H-thymidine concentration of 37 nM at which mouse embryo fibroblasts and calf aorta media cells respond with a dose-dependent stimulation of DNA synthesis, calf aorta endothelial cells only show a dose-dependent inhibition of the 3H-thymidine incorporation into the DNA. The relationship of this factor to the known growth factors and to the corpus luteum angiogenic factor (CLAF) deserves further investigation.
猪和牛的黄体含有一种活性物质，能够刺激小鼠胚胎成纤维细胞、小牛主动脉中膜细胞和小牛主动脉内皮细胞启动DNA合成。通过在CM-葡聚糖凝胶上进行离子交换色谱法，对硫酸铵逐步沉淀产生的提取物进行分级分离后，该活性在最后一个级分（峰5）中特别富集。然而，小牛主动脉内皮细胞的DNA合成起始强烈依赖于孵育培养基中标记胸腺嘧啶核苷的浓度。小牛主动脉内皮细胞仅在降低的3H-胸腺嘧啶核苷浓度9.25 nM时才对这种黄体因子有显著反应。在3H-胸腺嘧啶核苷浓度为37 nM时，小鼠胚胎成纤维细胞和小牛主动脉中膜细胞会以剂量依赖性方式刺激DNA合成，而小牛主动脉内皮细胞仅表现出3H-胸腺嘧啶核苷掺入DNA的剂量依赖性抑制。该因子与已知生长因子以及黄体血管生成因子（CLAF）的关系值得进一步研究。
