Sidorova E V, Agajanian M G, Korukova A A, Gurvich A E
Immunol Lett. 1981 Apr;3(1):21-6. doi: 10.1016/0165-2478(81)90090-0.
The cultivation of normal mouse spleen cells in a modified Mishell-Dutton system for 1-4 days in the presence of a water-soluble antigen of sheep red blood cells results in a sharp increase in the number of cells secreting non-specific immunoglobulins (nIFC). This increase is much more visible if spleen cells from mice primed with the same antigen 3-4 days before cultivation, are used. The rise in NIFC becomes apparent on day 1 and runs up to maximum on day 3. At this time a peak of 165 X 10(3) nIFC per 10(6) cells is attained, i.e. the nIFC quantity reaches approximately 33% of total B-cells. Kinetics of the antibody-forming cells and nIFC appearance under varying conditions is different. Clearcut differences are also revealed between the mechanisms of regulation of both these populations. The initial population of cells destined to form non-specific immunoglobulin is estimated to be 363/10(6) cells during a primary immune response in vitro; if splenocyte donors are primed with a homologous antigen, this population become approximately 800-1,900/10(6) cells.
在改良的米舍尔-达顿系统中，将正常小鼠脾细胞与绵羊红细胞水溶性抗原一起培养1 - 4天，会导致分泌非特异性免疫球蛋白的细胞（nIFC）数量急剧增加。如果使用在培养前3 - 4天用相同抗原致敏的小鼠脾细胞，这种增加会更加明显。NIFC的增加在第1天开始显现，并在第3天达到最大值。此时，每10⁶个细胞可达到165×10³个nIFC的峰值，即nIFC数量约占总B细胞的33%。在不同条件下，抗体形成细胞和nIFC出现的动力学是不同的。这两种细胞群体的调节机制也存在明显差异。在体外初次免疫反应期间，注定形成非特异性免疫球蛋白的初始细胞群体估计为每10⁶个细胞中有363个；如果脾细胞供体用同源抗原致敏，这个群体将变为约800 - 1900个/10⁶个细胞。
