Raedler E, Raedler A, Feldhaus S
Anat Embryol (Berl). 1981;162(1):21-8. doi: 10.1007/BF00318091.
The ability of prenatal cerebral tissue to bind different lectins was analyzed using cryostat sections of mouse brains. It was shown that the immature cells within the embryonic cell layers possess receptors for different lectins in varying amounts. Of all lectins tested, only PNL, RCL and LPL were bound on the outer cell membranes to a considerable degree. Although the labeling patterns of PNL and RCL are similar, the latter is additionally well detectable on the wall of cerebral blood vessels. Cells of the ventricular layer are moderately labeled by PNL, which recognizes beta-D-galactosyl (1-3)-N-acetyl-D-galactosamine, but heavily labeled by LPL which binds to terminal sialic acid residues. Cells of the intermediate layer, on the other hand, are heavily stained PNL and only faintly by LPL. Hence it is suggested that the process of migration might be correlated to the removal of terminal sialic acid moieties from cell surface glycoconjugates, resulting in an exposure of the penultimate galactosyl residues.
利用小鼠脑的冷冻切片分析了产前脑组织结合不同凝集素的能力。结果表明，胚胎细胞层内的未成熟细胞拥有数量各异的不同凝集素受体。在所有测试的凝集素中，只有PNL、RCL和LPL在细胞膜外有相当程度的结合。尽管PNL和RCL的标记模式相似，但RCL在脑血管壁上也能很好地检测到。室管膜层细胞被识别β-D-半乳糖基（1-3）-N-乙酰-D-半乳糖胺的PNL适度标记，但被结合末端唾液酸残基的LPL强烈标记。另一方面，中间层细胞被PNL强烈染色，被LPL微弱染色。因此，有人提出迁移过程可能与细胞表面糖缀合物末端唾液酸部分的去除有关，导致倒数第二个半乳糖基残基暴露。
