Buckley I K, Raju T R, Stewart M
J Cell Biol. 1981 Aug;90(2):309-11. doi: 10.1083/jcb.90.2.309.
Examination of sectioned, embedded material showed that almost all intermediate filaments normally present in cultured chick embryo fibroblasts, IMR-33 cells, and BHK-21 cells were eliminated by trypsinization. At the same time atypical filaments of intermediate size were formed by close apposition of thin filaments. Previous observations (Buckley et al., 1978, J. Cell Biol. 78:644-652) on negatively stained and on critical-point-dried whole-cell mounts confused these different filament types and, on the basis of heavy meromyosin binding to the atypical intermediate-sized filaments, we suggested that many normal intermediate filaments contain f-actin. The present results show that this suggestion was unwarranted and we therefore retract it.
对切片包埋材料的检查显示,培养的鸡胚成纤维细胞、IMR - 33细胞和BHK - 21细胞中正常存在的几乎所有中间丝都因胰蛋白酶处理而被清除。与此同时,细纤维紧密并列形成了中等大小的非典型丝。之前对负染和临界点干燥的全细胞装片的观察(Buckley等人,1978年,《细胞生物学杂志》78:644 - 652)混淆了这些不同的丝类型,并且基于重酶解肌球蛋白与非典型中等大小丝的结合,我们曾提出许多正常中间丝含有肌动蛋白丝。目前的结果表明这一推测是没有根据的,因此我们撤回该推测。