Dodin A, Plawecki M
Bull Soc Pathol Exot Filiales. 1978 Jan-Feb;71(1):22-33.
The Ch 1 + 2 fraction has been marked by means of culture of Vibrio cholerae Ogawa HK1 on a synthetic medium containing leucine H3. The antigen distribution has been then studied before and after vaccination with the same non-marked antigen in normal and axenic mice, at the same time by the demonstration of radioactivity and radioimmunofluorescence in intestine, spleen, liver, kidney and thymus. Whichever the administration route, intestine and spleen are first stimulated, then more intensively with the second injection or ingestion. When administrated per os the fraction crosses the intestinal barrier and is fixed on the main lymphoid organs: intestine, spleen, thymus. After ingestion or injection into axenic mice, spleen is the first organ stimulated.
通过在含有亮氨酸H3的合成培养基上培养霍乱弧菌小川型HK1来标记Ch 1 + 2组分。然后，在正常小鼠和无菌小鼠中，用相同的未标记抗原进行疫苗接种前后，通过在肠道、脾脏、肝脏、肾脏和胸腺中检测放射性和放射免疫荧光，同时研究抗原分布。无论给药途径如何，肠道和脾脏首先受到刺激，第二次注射或摄入后刺激更为强烈。当经口给药时，该组分穿过肠道屏障并固定在主要淋巴器官：肠道、脾脏、胸腺上。在无菌小鼠中摄入或注射后，脾脏是首先受到刺激的器官。
