Olansky A S, Deming S N
Clin Chem. 1978 Dec;24(12):2115-24.
A "stopped-flow" method for the kinetic Jaffé determination of creatinine was developed, with the use of a computer-controlled continuous-flow system. Simplex optimization was used to find conditions of hydroxide and picrate giving maximum sensitivity for creatinine. We used a modified central composite experimental design to evaluate creatinine sensitivity and albumin, glucose, and acetone interferences as functions of hydroxide and picrate concentrations. More importantly, this work illustrates that the automated development of clinical chemical methods offers an efficient means of obtaining optimized, well-understood analytical procedures for subsequent routine use in the clinical chemistry laboratory.
我们开发了一种用于肌酐动力学Jaffé测定的“停流”方法，采用计算机控制的连续流动系统。使用单纯形优化法来寻找能使肌酐灵敏度达到最高的氢氧化物和苦味酸盐条件。我们采用改进的中心复合实验设计来评估肌酐灵敏度以及白蛋白、葡萄糖和丙酮干扰与氢氧化物和苦味酸盐浓度的函数关系。更重要的是，这项工作表明临床化学方法的自动化开发为获得优化的、易于理解的分析程序提供了一种有效手段，以便随后在临床化学实验室中常规使用。
