Klumpp T, Férard G
Ann Biol Clin (Paris). 1977;35(3):203-8.
An automated kinetic method to determine creatinine in plasma with Jaffé reaction (alkaline picrate reagent) using a Gemsaec analyser is studied in order to argue the precision and the accuracy. The analysis of the reaction rate of a primar standard solution and of different plasmas suggests that a rapid measure is requisite in order to avoid interferences from chromogens contained in plasma which develop a same but often slower and durabler coloration at the used wavelength. The method is compared to the manual procedure using adsorption on Lloyd's reagent.
为了论证精密度和准确度，研究了一种使用Gemsaec分析仪通过Jaffé反应（碱性苦味酸盐试剂）测定血浆中肌酐的自动动力学方法。对一级标准溶液和不同血浆的反应速率分析表明，需要进行快速测量，以避免血浆中所含色原的干扰，这些色原在所用波长下会产生相同但通常较慢且更持久的颜色变化。将该方法与使用劳埃德试剂吸附的手动方法进行了比较。
