一种用于筛选淋巴细胞杂交瘤培养物中特异性抗体分泌的多价抗体放射免疫分析（MARIA）。
A multivalent antibody radioimmunoassay (MARIA) for screening specific antibody secretion by lymphocyte hybridoma cultures.
作者信息
Gheuens J, McFarlin D E
出版信息
J Immunol Methods. 1981;47(2):183-9. doi: 10.1016/0022-1759(81)90118-6.
A new radioimmunoassay which is suitable for screening lymphocyte hybridoma cultures for monoclonal antibody production is described. Solid-phase antigen is first incubated with the sample, and secondly with soluble 125I-labeled antigen. When the sample contains multivalent specific antibody, the labeled antigen will be bound to the solid-phase antigen. Because no anti-immunoglobulin reagent is used as a second reagent, this type of radioimmunoassay offers several advantages, in particular for the production of monoclonal anti-idiotypic antibodies.
本文描述了一种适用于筛选淋巴细胞杂交瘤培养物以生产单克隆抗体的新型放射免疫测定法。首先将固相抗原与样品孵育，其次与可溶性¹²⁵I标记抗原孵育。当样品含有多价特异性抗体时，标记抗原将与固相抗原结合。由于不使用抗免疫球蛋白试剂作为第二试剂，这种放射免疫测定法具有几个优点，特别是对于生产单克隆抗独特型抗体而言。
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