Vaidya H C, Dietzler D N, Ladenson J H
Hybridoma. 1985 Fall;4(3):271-6. doi: 10.1089/hyb.1985.4.271.
Hybridomas producing anti-creatine kinase (CK) and anti-lactate dehydrogenase (LDH) antibodies were screened by enzyme linked immunosorbant assay (ELISA) with antigen coated onto plastic wells. Out of seven antibodies positive for each isoenzyme of CK, four antibodies failed to bind to the radiolabeled antigen in solution phase radioimmunoassay (RIA) or native antigen in competitive ELISA. Moreover, out of nine antibodies shown reactive with LDH-1 and seven antibodies binding to LDH-5 by ELISA, not a single antibody bound to either radiolabeled or native antigen in solution. Our results along with other recent studies strongly suggest that antigen conformation on solid phase may frequently be different from in solution and that screening by ELISA in which antigen is attached to solid phase is often inappropriate for determining the presence of useful monoclonal antibodies.
通过酶联免疫吸附测定（ELISA），利用包被在塑料孔中的抗原筛选产生抗肌酸激酶（CK）和抗乳酸脱氢酶（LDH）抗体的杂交瘤。在对CK的每种同工酶呈阳性的七种抗体中，有四种抗体在溶液相放射免疫测定（RIA）中不能与放射性标记抗原结合，或在竞争性ELISA中不能与天然抗原结合。此外，在ELISA显示与LDH-1反应的九种抗体和与LDH-5结合的七种抗体中，没有一种抗体能与溶液中的放射性标记抗原或天然抗原结合。我们的结果以及其他近期研究强烈表明，固相上的抗原构象可能经常与溶液中的不同，并且通过将抗原附着于固相的ELISA进行筛选通常不适用于确定有用单克隆抗体的存在。
