Zenzes M T
Differentiation. 1981;18(3):169-73. doi: 10.1111/j.1432-0436.1981.tb01118.x.
Dissociation-reorganization experiments were done with gonadal cells of newborn rats. Rotation cultures consisted of mixtures of somatic and germ cells of opposite sex. Somatic cells, ovarian or testicular, determined a female or male type respectively, of gonadal histomorphic organization. Germ cells did not affect the type of organization of somatic cells. Accordingly, suspensions containing somatic cells of one sex together with germ cells of both sexes, reorganized in rotation culture, into either a) follicles containing XX or XY germ cells, or b) tubules containing XX or XY or both types of germ cells. These results give morphological evidence for heterosexual germ-somatic cells interactions. Based on morphological and H-Y antigen studies, failure of germ cells to bind and express H-Y antigen is considered as a possible factor for this failure of germ cells to affect gonadal sex.
对新生大鼠的性腺细胞进行了解离-重组实验。旋转培养由异性的体细胞和生殖细胞混合物组成。体细胞，无论是卵巢的还是睾丸的，分别决定了性腺组织形态组织的雌性或雄性类型。生殖细胞不影响体细胞的组织类型。因此，含有一种性别的体细胞与两种性别的生殖细胞的悬浮液，在旋转培养中重组为：a）含有XX或XY生殖细胞的卵泡，或b）含有XX或XY或两种类型生殖细胞的小管。这些结果为异性生殖细胞与体细胞的相互作用提供了形态学证据。基于形态学和H-Y抗原研究，生殖细胞未能结合并表达H-Y抗原被认为是生殖细胞影响性腺性别的这种失败的一个可能因素。
