Meyers K M, Hopkins G, Holmsen H, Benson K, Prieur D J
Am J Pathol. 1982 Mar;106(3):364-77.
The ultrastructural of platelets from Chédiak-Higashi (CH) and normal cattle, mink, and cats at rest was studied. Platelets from CH animals had a virtual absence of platelet dense granules. Alpha granules, amorphous membrane-surrounded structures, mitochondria, and microtubules of CH bovine platelets were similar in number and appearance to those in normal bovine platelets. Giant CH granules, present in other cells and considered diagnostic of the syndrome, could not be identified in platelets from CH animals. The open canalicular system and dense tubule system were not readily identifiable in resting bovine platelets. The ultrastructure of normal and CH cattle platelets was evaluated at various stages of ADP-induced aggregation. After platelets changed shape during the first phase of aggregation, the ultrastructural appearance of CH platelets was similar to that of normal platelets. The CH platelets composing the aggregates during irreversible aggregation did not appear as activated as did normal platelets, even though the aggregation tracings were similar. Normal and CH cattle platelets treated with thrombin appeared morphologically similar and were characterized by centrifugal movement of granules.
对处于静息状态的希德亚克-东综合征（CH）牛、水貂和猫以及正常牛、水貂和猫的血小板超微结构进行了研究。CH动物的血小板几乎没有血小板致密颗粒。CH牛血小板的α颗粒、无定形膜包围结构、线粒体和微管在数量和外观上与正常牛血小板相似。在其他细胞中出现且被认为是该综合征诊断依据的巨大CH颗粒，在CH动物的血小板中未被识别。在静息牛血小板中，开放管道系统和致密小管系统不易辨认。在ADP诱导的聚集的各个阶段评估了正常和CH牛血小板的超微结构。在聚集的第一阶段血小板改变形状后，CH血小板的超微结构外观与正常血小板相似。尽管聚集曲线相似，但在不可逆聚集过程中组成聚集体的CH血小板看起来并不像正常血小板那样活化。用凝血酶处理的正常和CH牛血小板在形态上相似，其特征是颗粒的离心运动。
