da Silva M B, Dessy C, Coghe J, David J L, Lekeux P
Department of Physiology, University of Liege, Belgium.
Vet Res Commun. 1998 Jun;22(4):273-91. doi: 10.1023/a:1006007802126.
The ultrastructure of bovine platelets was examined by transmission electron microscopy without any pretreatment (control), and after WEB 2086 (a triazolodiazepine) or ketoprofen (NSAID) pretreatment, followed by PAF infusion. The blood platelet count was also investigated. The group of calves that received WEB 2086 pretreatment before platelet-activating factor (PAF) infusion did not show a decreased number of platelets. However, in the other group, with ketoprofen pretreatment before PAF infusion, there was a rapid decrease from 1 to 3 min, while from 5 min the number of platelets recovered to the normal value. Electron microscopy revealed that pretreatment with WEB 2086 followed by PAF infusion did not alter the morphological ultrastructure of bovine platelets, except that the microtubules were briefly modified from 1 until 3 min after PAF challenge. After ketoprofen pretreatment, bovine platelets kept their regular shape, the number of dense bodies was not significantly altered, the number of mitochondria was maintained from 5 min after PAF infusion, giant platelets were not observed and the Golgi apparatus was rarely visible. Thus pretreatment with WEB 2086 and ketoprofen before PAF infusion had a protective activity on the ultrastructure of bovine platelets and, in cattle, pretreatment with WEB 2086 and ketoprofen before PAF challenge prevented the thrombocytopenia induced by PAF.
通过透射电子显微镜对未经任何预处理（对照）、经WEB 2086（一种三唑并二氮杂䓬）或酮洛芬（非甾体抗炎药）预处理后再输注血小板活化因子（PAF）的牛血小板超微结构进行了检查。同时也对血小板计数进行了研究。在输注PAF前接受WEB 2086预处理的小牛组血小板数量未减少。然而，在另一组中，在输注PAF前用酮洛芬预处理，血小板数量在1至3分钟内迅速下降，而从5分钟起血小板数量恢复到正常值。电子显微镜显示，经WEB 2086预处理后再输注PAF，除了在PAF激发后1至3分钟微管有短暂改变外，并未改变牛血小板的形态超微结构。经酮洛芬预处理后，牛血小板保持其正常形状，致密体数量无明显改变，从输注PAF后5分钟起线粒体数量维持不变，未观察到巨型血小板，高尔基体也很少可见。因此，在输注PAF前用WEB 2086和酮洛芬预处理对牛血小板超微结构具有保护作用，并且在牛中，在PAF激发前用WEB 2086和酮洛芬预处理可预防PAF诱导的血小板减少。
