人鼻旁黏膜组织型纤溶酶原激活剂与猪心组织型纤溶酶原激活剂理化性质的差异

Differences in physicochemical properties between tissue plasminogen activators from human paranasal mucous membrane and pig heart.

作者信息

Kosugi T, Takagi I, Matsuo O, Mihara H, Morimitsu T, Hirano K, Hamaya M

出版信息

Auris Nasus Larynx. 1981;8(2):77-86. doi: 10.1016/s0385-8146(81)80004-1.

PMID:7199907

Abstract

In order to clarify the physicochemical property of tissue plasminogen activator (TA), tissue extracts of human paranasal mucous membrane and pig heart were studied by the biochemical techniques. The studies by gel filtration revealed that two plasminogen activators of different molecular weight were present in the extract of human paranasal mucous membrane. The existence of tissue plasminogen activator with a low molecular weight (LMW-TA) has not previously been reported. This molecular weight of this compound was lower than that of cytochrome c. On the other hand, the molecular weight of tissue plasminogen activator from pig heart (PH-TA) was similar to that of ovalbumin, about 46,000 daltons, as estimated by Sephadex G-150 gel filtration. From the physicochemical property of LMW-TA, it is suggested that the LMW-TA from paranasal mucous membrane with chronic sinusitis was produced by proteases, i.e. trypsin-like enzyme, present in the mucous membrane with chronic inflammation.

摘要

为阐明组织型纤溶酶原激活剂（TA）的理化性质，采用生化技术对人鼻旁黏膜和猪心的组织提取物进行了研究。凝胶过滤研究表明，人鼻旁黏膜提取物中存在两种分子量不同的纤溶酶原激活剂。此前尚未报道过低分子量组织型纤溶酶原激活剂（LMW-TA）的存在。该化合物的分子量低于细胞色素c。另一方面，通过Sephadex G-150凝胶过滤估计，猪心组织型纤溶酶原激活剂（PH-TA）的分子量与卵清蛋白相似，约为46,000道尔顿。从LMW-TA的理化性质推测，慢性鼻窦炎鼻旁黏膜中的LMW-TA是由慢性炎症黏膜中存在的蛋白酶即类胰蛋白酶产生的。