Forsell J H, Shull L R, Kateley J R
J Toxicol Environ Health. 1981 Oct;8(4):543-58. doi: 10.1080/15287398109530090.
Eight lactating Holstein-Friesian dairy cattle were randomly allotted as pairs to eight a control or a treatment group fed technical pentachlorophenol (penta) from 6 +/-1 wk postpartum for about 135 d (0.2 mg/kg.d for 75-84 d followed by 2.0 mg/kg.d for 56-62 d). Jugular blood was drawn periodically for immunologic studies. Leukocyte differentials and lymphocyte subpopulations (e.g., T and B cells) were enumerated for each blood specimen. Several in vitro and in vivo assays were conducted to evaluate lymphocyte functions, including (1) quantitation of serum immunoglobulins G, M, and A; (2) induction of blastogenesis in vitro, using concanavalin A and leukoagglutinin as mitogens; (3) measurement of extent of skin reaction to injected purified protein derivative in BCG-sensitized cattle to evaluate delayed hypersensitivity; and (4) quantitation of antibody formation in response to injected sheep red blood cells. Neutrophil function was evaluated by latex particle phagocytosis and chemiluminescence. The results showed no statistically significant differences between control and pent-treated cattle during eigher treatment period. Also, no histopathologic changes were noted in lymphoid tissues including spleen, thymus, and lymph node.
八头泌乳期的荷斯坦-弗里生奶牛被随机配对分为对照组和处理组，从产后6±1周开始给处理组饲喂工业五氯苯酚（五氯酚），持续约135天（75 - 84天为0.2毫克/千克·天，随后56 - 62天为2.0毫克/千克·天）。定期采集颈静脉血用于免疫学研究。对每份血液样本进行白细胞分类计数和淋巴细胞亚群（如T细胞和B细胞）计数。进行了多项体外和体内试验以评估淋巴细胞功能，包括：（1）定量血清免疫球蛋白G、M和A；（2）以刀豆球蛋白A和白细胞凝集素作为有丝分裂原，体外诱导细胞增殖；（3）测量卡介苗致敏牛对注射纯化蛋白衍生物的皮肤反应程度，以评估迟发型超敏反应；（4）定量对注射绵羊红细胞产生的抗体形成。通过乳胶颗粒吞噬作用和化学发光评估中性粒细胞功能。结果显示，在两个处理期内，对照组和五氯酚处理组奶牛之间均无统计学上的显著差异。此外，在包括脾脏、胸腺和淋巴结在内的淋巴组织中未观察到组织病理学变化。
