Fraser L R
J Reprod Fertil. 1982 May;65(1):185-94. doi: 10.1530/jrf.0.0650185.
The effect of p-aminobenzamidine (pAB), an inhibitor of mouse sperm acrosin, on mouse sperm capacitation, motility, acrosome loss and fertility in vitro was examined using zona-intact and zona-free eggs. With intact eggs, concentrations of pAB ranging from 0.1 to 1.0 mM in the sperm preincubation medium effectively inhibited fertilization (13-0%, respectively), but these same suspensions (10(6) cells/ml) showed high rates of fertilization with zona-free eggs (100-95.3%); with the lower concentration of 10(5) cells/ml, fertilization rates of zona-free eggs decreased with increasing concentrations of pAB (100-55%). Washing of treated samples gave fertilization rates similar to control samples (87.1 and 84.6%, respectively), indicating that inhibition was reversible and that there had been no interference with the capacitation process. Whiplash motility was also observed in all samples, suggesting that the apparent inability to penetrate the zona might be due to an acrosomal defect. This was confirmed by electron microscopic examination of treated sperm samples. In high concentrations of pAB, many cells had undergone the acrosome reaction, i.e. membrane vesiculation, but acrosomal matrix dispersal was inhibited. These results are consistent, therefore, with a role for the acrosomal enzyme acrosin in matrix dispersal, but not the acrosome reaction itself, and in penetration of the zona pellucida.
使用有透明带和无透明带的卵子，研究了小鼠精子顶体蛋白酶抑制剂对氨基苯甲脒（pAB）在体外对小鼠精子获能、活力、顶体丢失和受精能力的影响。对于有透明带的卵子，精子预孵育培养基中pAB浓度在0.1至1.0 mM范围内可有效抑制受精（分别为13%至0%），但这些相同的悬浮液（10⁶个细胞/毫升）与无透明带的卵子受精率较高（100%至95.3%）；对于较低浓度10⁵个细胞/毫升，无透明带卵子的受精率随pAB浓度增加而降低（100%至55%）。处理过的样本洗涤后受精率与对照样本相似（分别为87.1%和84.6%），表明抑制是可逆的，且对获能过程无干扰。在所有样本中也观察到了鞭打样运动，这表明明显无法穿透透明带可能是由于顶体缺陷。对处理过的精子样本进行电子显微镜检查证实了这一点。在高浓度pAB中，许多细胞发生了顶体反应，即膜泡化，但顶体基质扩散受到抑制。因此，这些结果与顶体酶顶体蛋白酶在基质扩散而非顶体反应本身以及穿透透明带中的作用一致。
