Martin D H, Dietz W H, Alvaerez O, Johnson K M
Am J Trop Med Hyg. 1982 May;31(3 Pt 1):561-8. doi: 10.4269/ajtmh.1982.31.561.
One hundred and fifty-eight strains of Venezuelan equine encephalomyelitis virus were typed antigenically and classified epidemiologically as either epizootic or enzootic. Plaque sizes for 148 of these strains were determined, and the pH requirements for hemagglutination (HA) of goose erythrocytes of 131 were studied. Only antigenic variant group IABC strains could be classified epidemiologically as epizootic. In vitro these strains were characterized by the formation of small plaques in Vero cells and a relatively narrow pH range for optimum HA reactivity. Experimental studies in horses confirmed the fact that only IABC strains have epizootic potential. We concluded that plaque size in Vero cell monolayers would be a useful method of screening VEE viruses for equine virulent strains. Indirect evidence suggested that small plaques resulted from sensitivity to an anionic substance present in the agar overlay medium.
对158株委内瑞拉马脑炎病毒进行了抗原分型，并根据流行病学将其分为 epizootic 或 enzootic 类型。测定了其中148株病毒的蚀斑大小，并研究了131株病毒对鹅红细胞血凝（HA）的pH要求。只有抗原变异组IABC株在流行病学上可归类为 epizootic。在体外，这些毒株的特征是在Vero细胞中形成小蚀斑，且HA反应的最佳pH范围相对较窄。在马身上进行的实验研究证实，只有IABC株具有 epizootic 潜力。我们得出结论，Vero细胞单层中的蚀斑大小将是筛选马脑炎病毒强毒株的一种有用方法。间接证据表明，小蚀斑是由于对琼脂覆盖培养基中存在的一种阴离子物质敏感所致。
