Pradalier N, Canal P, Pujol A, Fregevu Y, Soula G
Ann Biol Clin (Paris). 1982;40(1):45-50.
We propose a double antibody radioimmunoassay for human prostatic acid phosphatase (PAP) in serum for diagnosis and management of prostatic adenocarcinoma under treatment. The antigen is purified from human prostatic fluid by a gel-filtration on Sephadex G 100 followed by affinity chromatography on Con A Sepharose. A specific antibody is raised in rabbits and purified by immunoadsorption with a female serum. The described technique offers both radioisotopic sensibility and immunologic specificity. Physiological values determined in the serum of 125 healthy males are below 2 ng/ml. No significative differences are observed with age. The proposed technique also shows significant differences between values evaluated for benign prostatic hyperplasia and prostatic adenocarcinoma.
我们提出一种用于血清中人前列腺酸性磷酸酶（PAP）的双抗体放射免疫测定法，用于前列腺腺癌治疗过程中的诊断和管理。抗原从人前列腺液中通过在Sephadex G 100上的凝胶过滤，然后在Con A Sepharose上进行亲和层析来纯化。用兔制备特异性抗体，并用女性血清通过免疫吸附进行纯化。所描述的技术兼具放射性同位素敏感性和免疫特异性。在125名健康男性血清中测定的生理值低于2 ng/ml。未观察到随年龄有显著差异。所提出的技术在良性前列腺增生和前列腺腺癌评估值之间也显示出显著差异。
