Morikawa J, Nakamura M, Mori K, Ohsawa R, Miki M, Machida T
Radioisotopes. 1980 Apr;29(4):175-80. doi: 10.3769/radioisotopes.29.4_175.
We purified human prostatic acid phosphatase (hPAP) from prostatic tissues by affinity chromatography, DEAE cellulose and gel filtration and also examined physicochemical properties of highly purified PAP. We developed a double-antibody radioimmunoassay for hPAP in serum, with use of antiserum raised in rabbit against highly purified PAP. The antiserum did not cross react with acid phosphatase from platelets and red blood cells. Experimental detail are outlined to assess the reproducibility and reliability of the method under various conditions. The upper limit of the serum PAP levels in the present assay was set at 3.0 ng/ml by 162 determinations of samples. The serum PAP levels of 2 untreated patients with prostatic carcinoma were higher than 3.0 ng/ml and 39 patients with benign prostatic hyperplasia were an average value of 1.9 ng/ml.
我们通过亲和层析、DEAE纤维素和凝胶过滤从前列腺组织中纯化了人前列腺酸性磷酸酶（hPAP），并研究了高度纯化的PAP的理化性质。我们使用针对高度纯化的PAP在兔体内产生的抗血清，开发了一种用于检测血清中hPAP的双抗体放射免疫测定法。该抗血清与血小板和红细胞中的酸性磷酸酶无交叉反应。概述了实验细节，以评估该方法在各种条件下的重现性和可靠性。通过对162份样本的测定，本测定中血清PAP水平的上限设定为3.0 ng/ml。2例未经治疗的前列腺癌患者的血清PAP水平高于3.0 ng/ml，39例良性前列腺增生患者的血清PAP水平平均值为1.9 ng/ml。
