Boneu B, Robert A, Sie P, Elkoury M, Liverato C, Caranobe C, Nouvel C
Br J Haematol. 1982 Jun;51(2):305-11.
The addition of distilled water to platelet rich plasma converts the platelets into large spherocytes without lysis. This phenomenon implies an evagination of the platelet membrane which participates in the surface connecting system. An adaptation of the direct microscopic method (Milton & Frojmovic, 1979) to the Coulter Counter is reported. The maximum increase in platelet volume without platelet lysis was obtained for 120 mOsm. These volume changes are a consequence of a shift in the platelet volume distribution curve towards higher values without loss of log normality, while the volume dispersion is only slightly modified. This suggests that the amount of evaginable platelet membrane is correlated to the size of the platelet. The size parameters (volume, surface, diameter) of hypotonic-induced spherocytes closely agree with those reported in the original microscopic method. Application of this technique to ITP-platelets suggests a reduced evaginable platelet membrane; this fact probably reflects the abnormal demarcation of platelet territories at the megakaryocytic level.
向富含血小板的血浆中加入蒸馏水会使血小板转化为大球形细胞而不发生裂解。这种现象意味着血小板膜的外翻，其参与表面连接系统。本文报道了一种将直接显微镜法（米尔顿和弗罗伊莫维奇，1979年）应用于库尔特计数器的方法。在120毫渗量浓度下可获得无血小板裂解时血小板体积的最大增加。这些体积变化是血小板体积分布曲线向更高值移动的结果，且对数正态性未丧失，而体积离散度仅略有改变。这表明可外翻的血小板膜量与血小板大小相关。低渗诱导的球形细胞的大小参数（体积、表面积、直径）与原始显微镜法报道的参数非常一致。将该技术应用于特发性血小板减少性紫癜患者的血小板表明可外翻的血小板膜减少；这一事实可能反映了巨核细胞水平上血小板区域的异常划分。
