Illes I, Pfueller S L, Hussein S, Chesterman C N, Martin J F
Department of Medicine, University of Melbourne, St Vincent's Hospital, Victoria, Australia.
Br J Haematol. 1987 Oct;67(2):173-6. doi: 10.1111/j.1365-2141.1987.tb02322.x.
To determine whether platelet size and volume are related to one another or to platelet age, subpopulations of platelets from patients with idiopathic thrombocytopenic purpura (ITP) have been produced on the basis of density using Percoll gradients. The density distribution of platelets from patients with ITP and from patients with other forms of thrombocytopenia (thought to be nonimmune in nature) was the same as in normal controls. However, the platelets in each density subpopulation from ITP patients were increased in size. beta-thromboglobulin (beta TG) content of platelets from each patient group and the normals increased with density and tended to be higher in ITP than in normal controls. beta TG concentration per unit platelet volume and its level in plasma were similar in ITP patients and in normal controls. This suggests that the apparently normal density of ITP platelets was not a result of degranulation of large, dense platelets. Thus platelet size and density are independently determined and the increased size of platelets in immune thrombocytopenia may be the result of abnormalities in their production.
为了确定血小板大小和体积是否相互关联或与血小板年龄有关，已利用Percoll梯度，根据密度对特发性血小板减少性紫癜（ITP）患者的血小板亚群进行了划分。ITP患者以及其他形式血小板减少症（被认为本质上是非免疫性的）患者的血小板密度分布与正常对照组相同。然而，ITP患者每个密度亚群中的血小板大小均有所增加。各患者组和正常对照组血小板的β-血小板球蛋白（βTG）含量均随密度增加，且ITP患者的含量往往高于正常对照组。ITP患者和正常对照组每单位血小板体积的βTG浓度及其血浆水平相似。这表明ITP血小板看似正常的密度并非大的致密血小板脱颗粒的结果。因此，血小板大小和密度是独立决定的，免疫性血小板减少症中血小板大小增加可能是其生成异常的结果。
