Thirion J P, Aw E J
Can J Microbiol. 1982 Mar;28(3):356-9. doi: 10.1139/m82-053.
Somatic cell variants of Chinese hamster V79 were selected for rapid growth on D-galactose at high temperatures. Their phenotypes are stable after many generations in the absence of selection pressure. They clone in D-galactose at temperatures at which the wild type cannot, while in D-glucose, the variants and the wild type appear to have the same growth properties. The use of such variants should be very important for the study of gene regulation of carbohydrate metabolism in mammalian cells.
中国仓鼠V79的体细胞变体是通过在高温下于D-半乳糖上快速生长而筛选出来的。在没有选择压力的情况下，经过许多代后它们的表型依然稳定。它们能够在野生型无法生长的温度下于D-半乳糖中克隆，而在D-葡萄糖中，变体和野生型似乎具有相同的生长特性。这类变体的应用对于研究哺乳动物细胞中碳水化合物代谢的基因调控应该非常重要。
