Thirion J P, Banville D, Noel H
Genetics. 1976 May;83(1):137-147. doi: 10.1093/genetics/83.1.137.
The growth of Chinese hamster somatic cells was inhibited by 0.2 mg/cc of 2-deoxygalactose. Mutants partially or fully resistant to 2-deoxygalactose were isolated in a single-step or two-step selection. Some of them did not grow as well as the wild type; one of them which lacked galactokinase(EC.2.7.1.6) activity did not grow at all in galactose medium. The galactokinase kinetic properties (Vmax & kmax of the other mutants and of the wild type were different. Therefore resistance resulted either from the possible absence of galactokinase synthesis or from a structural mutation, possible a missence mutation, in the galactokinase gene.- A simple diagnostic test for juvenile cataract is proposed.
0.2毫克/立方厘米的2-脱氧半乳糖可抑制中国仓鼠体细胞的生长。通过一步或两步筛选分离出了对2-脱氧半乳糖部分或完全抗性的突变体。其中一些突变体的生长情况不如野生型；其中一个缺乏半乳糖激酶（EC.2.7.1.6）活性的突变体在半乳糖培养基中根本无法生长。其他突变体和野生型的半乳糖激酶动力学特性（Vmax和kmax）不同。因此，抗性可能是由于半乳糖激酶合成缺失，或者是由于半乳糖激酶基因发生了结构突变，可能是错义突变。——提出了一种针对青少年白内障的简单诊断测试。
