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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Fujii M, Yabu Y, Fukunaga T, Koga K

Int J Biochem. 1982;14(5):355-7. doi: 10.1016/0020-711x(82)90018-0.

The amount of lipid decreased markedly at the later stage of embryonic development, coinciding with the remarkable decrease in yolk weight. 2. Phospholipase C activity in the yolk sac appeared on the 6th day of incubation and increased markedly as incubation proceeded. 3. The enzyme showed its optimum activity at pH 9.5 and at 40 degrees C. 4. The activity was enhanced 2-fold by 20 mM CaCl2 and by 40% by 4 mM deoxycholate but inhibited by 2 mM ZnCl2 and CuSO4.

在胚胎发育后期，脂质含量显著下降，这与卵黄重量的显著减少相一致。2. 卵黄囊中磷脂酶C活性在孵化第6天出现，并随着孵化进程显著增加。3. 该酶在pH 9.5和40摄氏度时表现出最佳活性。4. 20 mM氯化钙可使活性增强2倍，4 mM脱氧胆酸盐可使活性增强40%，但2 mM氯化锌和硫酸铜会抑制其活性。

Fujii M, Yabu Y, Fukunaga T, Koga K

Int J Biochem. 1982;14(5):355-7. doi: 10.1016/0020-711x(82)90018-0.

The amount of lipid decreased markedly at the later stage of embryonic development, coinciding with the remarkable decrease in yolk weight. 2. Phospholipase C activity in the yolk sac appeared on the 6th day of incubation and increased markedly as incubation proceeded. 3. The enzyme showed its optimum activity at pH 9.5 and at 40 degrees C. 4. The activity was enhanced 2-fold by 20 mM CaCl2 and by 40% by 4 mM deoxycholate but inhibited by 2 mM ZnCl2 and CuSO4.

在胚胎发育后期，脂质含量显著下降，这与卵黄重量的显著减少相一致。2. 卵黄囊中磷脂酶C活性在孵化第6天出现，并随着孵化进程显著增加。3. 该酶在pH 9.5和40摄氏度时表现出最佳活性。4. 20 mM氯化钙可使活性增强2倍，4 mM脱氧胆酸盐可使活性增强40%，但2 mM氯化锌和硫酸铜会抑制其活性。