底物对冻干及投影蛋白结构的影响。
Effect of substrate on freeze-dried and shadowed protein structures.
作者信息
Trinick J, Elliott A
出版信息
J Microsc. 1982 May;126(Pt 2):151-6. doi: 10.1111/j.1365-2818.1982.tb00364.x.
Abstract
Muscle thick filaments have been prepared for electron microscopy by a method involving freeze-drying and shadowing. The same method applied to filaments on three different substrates gives rise to quite different appearances. The filaments on one of the substrates, hydrophilic carbon, show new detail not previously observed.
摘要
已通过一种涉及冷冻干燥和投影的方法制备了用于电子显微镜观察的肌肉粗丝。将相同方法应用于三种不同底物上的细丝会产生截然不同的外观。其中一种底物亲水碳上的细丝呈现出以前未观察到的新细节。
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