Parks O W
J Assoc Off Anal Chem. 1982 May;65(3):632-4.
A screening method is described for estimation of sulfamethazine and sulfathiazole residues in swine livers. Tissues are homogenized with CHCl3-ethyl acetate (1 + 1). The drugs are extracted from the organic solvent with pH 10 carbonate buffer and back-extracted into dichloromethane, as an ion-pair with tetrabutylammonium hydroxide, without pH adjustment. Following evaporation of the solvent, the residue is dissolved in methanol, subjected to thin layer chromatography, and detected by the Bratton-Marshall reaction. Recoveries of sulfamethazine and sulfathiazole, determined by high pressure liquid chromatography, were 50.8 and 42%, respectively, with coefficients of variation of 4.2 and 4.7%.
本文描述了一种用于估算猪肝中磺胺二甲嘧啶和磺胺噻唑残留量的筛选方法。将组织用三氯甲烷 - 乙酸乙酯（1 + 1）匀浆。药物从有机溶剂中用pH 10的碳酸盐缓冲液萃取，然后在不调节pH的情况下，作为与氢氧化四丁铵的离子对反萃取到二氯甲烷中。溶剂蒸发后，残留物溶解在甲醇中，进行薄层色谱分析，并通过布拉顿 - 马歇尔反应进行检测。通过高压液相色谱法测定，磺胺二甲嘧啶和磺胺噻唑的回收率分别为50.8%和42%，变异系数分别为4.2%和4.7%。
