McKeehan W L, Glass H A, Rosser M P, Adams P S
Prostate. 1982;3(3):231-46. doi: 10.1002/pros.2990030306.
Prostatic binding protein (PBP), polyamine, and DNA synthesis were examined in primary cultures of rat ventral prostate cells. Soon after aggregates of prostate cells were placed in culture, PBP synthesis fell dramatically and DNA synthetic activity in the epithelial cells increased. The amount of polyamines that were labeled with [3H] from [3H] ornithine fell transiently, but rose again at or before the peak of DNA synthesis. Individual 3H-labeled polyamines in cells and medium were dansylated and separated by thin-layer chromatography. The ratio of [3H] Spermidine plus [3H] Spermine to [3H] Putrescine in the culture medium declined as DNA synthesis increased. Ornithine decarboxylase (ODC) activity fell dramatically along with PBP synthesis even as DNA synthesis and 3H-labeled polyamines increased in the prostate-cell cultures. These results support others that suggest that high ODC activity in prostate epithelial cells is a correlate of prostate epithelial cell function rather than proliferation. However, prostate epithelial cells retain the capacity to synthesize significant levels of polyamines from ornithine (especially Putrescine) during proliferation even when ODC activity is low.
在大鼠腹侧前列腺细胞原代培养物中检测了前列腺结合蛋白（PBP）、多胺和DNA合成。前列腺细胞聚集体置于培养后不久，PBP合成急剧下降，上皮细胞中的DNA合成活性增加。用[3H]鸟氨酸标记的多胺量短暂下降，但在DNA合成达到峰值时或之前再次上升。细胞和培养基中单个3H标记的多胺用丹磺酰化，并通过薄层色谱法分离。随着DNA合成增加，培养基中[3H]亚精胺加[3H]精胺与[3H]腐胺的比例下降。即使在前列腺细胞培养物中DNA合成和3H标记的多胺增加时，鸟氨酸脱羧酶（ODC）活性也随着PBP合成急剧下降。这些结果支持了其他研究结果，表明前列腺上皮细胞中高ODC活性是前列腺上皮细胞功能而非增殖的一个相关因素。然而，即使ODC活性较低，前列腺上皮细胞在增殖过程中仍保留从鸟氨酸（尤其是腐胺）合成大量多胺的能力。
