Hinkley R E, Chambers E L
J Cell Sci. 1982 Jun;55:327-39. doi: 10.1242/jcs.55.1.327.
Fertilized Lytechinus eggs exposed to the volatile anaesthetic halothane before metaphase do not undergo cytoplasmic cleavage. This effect has been correlated with the failure of the contractile ring to assemble. Comparative studies on mitotic apparatuses isolated from control and halothane-treated cells show that halothane significantly impairs both spindle and aster growth as early as metaphase. When transferred to control solutions, halothane-treated cells initiate furrowing activity in association with either the first or second mitotic division, depending on the duration of the exposure to anaesthetic and the concentration employed. In contrast to these effects, halothane has no effect on any aspect of the cleavage process if applied later than metaphase. In this case, furrows develop even in the presence of halothane, deepen progressively, and complete cell division. These observations confirm and extend previous studies on echinoderm eggs exposed to volatile anaesthetics, and support the view that anaesthetic agents indirectly prevent cell cleavage by inhibiting the growth of mitotic apparatus.
在中期之前暴露于挥发性麻醉剂氟烷的受精海胆卵不会发生细胞质分裂。这种效应与收缩环无法组装有关。对从对照细胞和经氟烷处理的细胞中分离出的有丝分裂装置进行的比较研究表明，早在中期，氟烷就会显著损害纺锤体和星体的生长。当转移到对照溶液中时，经氟烷处理的细胞会根据暴露于麻醉剂的持续时间和所用浓度，在第一次或第二次有丝分裂时开始沟裂活动。与这些效应相反，如果在中期之后应用氟烷，则对分裂过程的任何方面都没有影响。在这种情况下，即使存在氟烷，沟裂也会形成，逐渐加深，并完成细胞分裂。这些观察结果证实并扩展了先前关于暴露于挥发性麻醉剂的棘皮动物卵的研究，并支持麻醉剂通过抑制有丝分裂装置的生长间接阻止细胞分裂的观点。
