Wakayama Y, Ohbu S
J Neurol Sci. 1982 Jul;55(1):59-77. doi: 10.1016/0022-510x(82)90170-8.
Light-microscopic study of muscles from 8 patients with Duchenne muscular dystrophy (DMD) and 9 control cases transplanted into nude mice showed the formation of cross-striations and peripheral nuclei in regenerating muscle fibers of both DMD and control grafts up to 4 weeks. Quantitative study of the diameter of myotubes and myoblasts in grafted muscles showed that the group mean diameters of DMD myotubes and myoblasts were 13.4 +/- 0.8 (SE) micrometers, 15.6 +/- 0.7 micrometers, 17.1 +/- 0.9 micrometers and 16.2 +/- 1.0 micrometers after 1, 2, 3 and 4 weeks of transplantation, respectively, whereas those of control myotubes and myoblasts were 14.9 +/- 0.6 (SE) micrometers, 19.9 +/- 0.5 micrometers, 21.3 +/- 0.7 micrometers and 20.7 +/- 0.7 micrometers after the same intervals. The group mean diameters of the DMD regenerating myotubes and myoblasts were significantly smaller than those of control myotubes and myoblasts at 2 (P less than 0.001), 3 (P less than 0.005) and 4 (P less than 0.005) weeks after transplantation. Electron-microscopic findings of DMD muscle grafts were morphologically similar to those seen in control muscle grafts at all stages of this experiment.
对8例杜氏肌营养不良症（DMD）患者和9例对照者的肌肉移植到裸鼠后的光镜研究显示，在长达4周的时间里，DMD和对照移植组织的再生肌纤维中均形成了横纹和外周核。对移植肌肉中肌管和成肌细胞直径的定量研究表明，移植后1、2、3和4周，DMD肌管和成肌细胞的组平均直径分别为13.4±0.8（SE）微米、15.6±0.7微米、17.1±0.9微米和16.2±1.0微米，而对照肌管和成肌细胞在相同时间间隔后的直径分别为14.9±0.6（SE）微米、19.9±0.5微米、21.3±0.7微米和20.7±0.7微米。移植后2周（P＜0.001）、3周（P＜0.005）和4周（P＜0.005）时，DMD再生肌管和成肌细胞的组平均直径显著小于对照肌管和成肌细胞。在本实验的所有阶段，DMD肌肉移植组织的电镜观察结果在形态上与对照肌肉移植组织相似。
